Anti-RUNX1 / AML1 antibody - ChIP Grade
| Name | Anti-RUNX1 / AML1 antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab23980 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ChIP EMSA WB ChIPseq ICC/IF ICC/IF IP |
| Species Reactivities | Rat, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Runx1/AML1 |
| Description | Rabbit Polyclonal |
| Gene | RUNX1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-RUNX1 / AML1 antibody - ChIP Grade (ab23980) at 1/1000 dilutionLane 1 : Mouse BladderLane 2 : Mouse LungLane 3 : Mouse LiverLane 4 : Mouse ThymusSecondaryDonkey polyclonal to anti-Rabbit IgG, HRP-Linked F(ab')2 Fragment at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Anti-RUNX1 / AML1 antibody - ChIP Grade (ab23980) at 1 µg/ml + Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844) at 20 µgSecondaryRabbit IgG secondary antibody (ab28446) at 1/10000 dilution
All lanes : Anti-RUNX1 / AML1 antibody - ChIP Grade (ab23980) at 1 µg/mlLane 1 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell LysateLane 2 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)Lane 3 : Thymus (Mouse) Tissue LysateLane 4 : Thymus (Rat) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Chromatin was prepared from nuclear lysate of the mouse haematopoietic progenitor cell line HPC-7. The cross-linking (X-ChiP) technique was used, crosslinking was performed for 10 minutes in 1% formaldehyde. The primary antibody was used undiluted and incubated with the sample for 16 hours at 4°C. The immunoprecipitated DNA was quantified by real time PCR. Negative control: A regultory element which is an open region of chromatin but is devoid of this transcription factor.See Abreview
ab23980 staining RUNX1 / AML1 in human glioblastoma cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton X-100 and then blocked using 0.5% BSA for 20 minutes. Samples were then incubated with primary antibody at 1/50 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Cy3® used at a 1/400 dilution.
Product References
NOTCH1-RBPJ complexes drive target gene expression through dynamic interactions - NOTCH1-RBPJ complexes drive target gene expression through dynamic interactions
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Ezh2 loss promotes development of myelodysplastic syndrome but attenuates its - Ezh2 loss promotes development of myelodysplastic syndrome but attenuates its
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Deletion of core-binding factor beta (Cbfbeta) in mesenchymal progenitor cells - Deletion of core-binding factor beta (Cbfbeta) in mesenchymal progenitor cells
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RUNX1-dependent RAG1 deposition instigates human TCR-delta locus rearrangement. - RUNX1-dependent RAG1 deposition instigates human TCR-delta locus rearrangement.
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Microglia emerge from erythromyeloid precursors via Pu.1- and Irf8-dependent - Microglia emerge from erythromyeloid precursors via Pu.1- and Irf8-dependent
Kierdorf K, Erny D, Goldmann T, Sander V, Schulz C, Perdiguero EG, Wieghofer P, Heinrich A, Riemke P, Holscher C, Muller DN, Luckow B, Brocker T, Debowski K, Fritz G, Opdenakker G, Diefenbach A, Biber K, Heikenwalder M, Geissmann F, Rosenbauer F, Prinz M. Nat Neurosci. 2013 Mar;16(3):273-80.
The control of hematopoietic stem cell maintenance, self-renewal, and - The control of hematopoietic stem cell maintenance, self-renewal, and
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Runx1 deficiency permits granulocyte lineage commitment but impairs subsequent - Runx1 deficiency permits granulocyte lineage commitment but impairs subsequent
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