Anti-RUNX2 antibody
| Name | Anti-RUNX2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab114133 |
| Prices | $376.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Human, Mouse, Rat, Rabbit, Chimpanzee, Monkey, Ape, Hamster, Orangutan |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human RUNX2 |
| Description | Rabbit Polyclonal |
| Gene | RUNX2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab114133 stained U20S cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab114133, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-RUNX2 antibody (ab114133) at 1 µg/ml + Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.