Anti-RUNX2 antibody
| Name | Anti-RUNX2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab135674 |
| Prices | $384.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB FC |
| Species Reactivities | Human |
| Antigen | Synthetic peptide conjugated to KLH, corresponding to a region within amino acids 512-541 (surrounding S533) of Human RUNX2 |
| Description | Rabbit Polyclonal |
| Gene | RUNX2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of RUNX2 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab135674, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Anti-RUNX2 antibody (ab135674) at 1/50 dilution + HepG2 cell line lysate at 35 µg
Anti-RUNX2 antibody (ab135674) at 1/50 dilution + Hob cell line lysate
Flow cytometric analysis of NCI-H460 cells labelling RUNX2 with ab135674 at 1/10 dilution (green) compared to negative control cells (blue). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.