All lanes : Anti-SDHB antibody [EPR13042(B)] (ab178423) at 1/1000 dilutionLane 1 : Human fetal liver lysatesLane 2 : HepG2 lysatesLane 3 : A431 lysatesLane 4 : Jurkat lysatesLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution
Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling SDHB with ab178423 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SDHB with ab178423 at 1/50 dilution.
Flow Cytometric analysis of permeabilized HepG2 cells labeling SDHB with ab178423 at 1/10 dilution (red) compared with a rabbit IgG negative control (green).
Western blot analysis on immunoprecipitation pellet from (1) A431 cell lysate or (2) 1X PBS (negative control) immunoprecipitated using ab178423 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.