Anti-SERCA2 ATPase antibody
| Name | Anti-SERCA2 ATPase antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab3625 |
| Prices | $400.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F IP WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Mouse, Rat, Rabbit, Human, Pig, Horse, Chicken, Guinea Pig, Bovine, Dog, Turkey, Xenopus, Chimpanzee, Monkey, Ape, Orangutan, Xenopus, Platypus |
| Antigen | Synthetic peptide - which represents a portion of Sarcoplasmic/ Endoplasmic Reticulum Calcium ATPase 2 (SERCA 2)encoded within exon 12 |
| Description | Rabbit Polyclonal |
| Gene | ATP2A2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Ab3625 staining human heart. Staining is localised to the cytoplasm/sarcoplasm.Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
ICC/IF image of ab3625 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3625, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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