![All lanes : Anti-SHC (phospho Y239 + Y240) antibody [EP419] (ab109455) at 1/5000 dilutionLane 1 : 293T cell lysates, EGF treated with single phospho (pY239) peptideLane 2 : 293T cell lysates, EGF treated with single phospho (pY240) peptideLane 3 : 293T cell lysates, EGF treated with Double phospho (pY239/pY240) peptideLane 4 : 293T cell lysates, EGF treated with non-phospho peptide blockingLane 5 : 293T cell lysates, EGF treatedLysates/proteins at 10 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/29650_ab109455-213860-ab109455.jpg)
All lanes : Anti-SHC (phospho Y239 + Y240) antibody [EP419] (ab109455) at 1/5000 dilutionLane 1 : 293T cell lysates, EGF treated with single phospho (pY239) peptideLane 2 : 293T cell lysates, EGF treated with single phospho (pY240) peptideLane 3 : 293T cell lysates, EGF treated with Double phospho (pY239/pY240) peptideLane 4 : 293T cell lysates, EGF treated with non-phospho peptide blockingLane 5 : 293T cell lysates, EGF treatedLysates/proteins at 10 µg per lane.
![All lanes : Anti-SHC (phospho Y239 + Y240) antibody [EP419] (ab109455) at 1/500 dilutionLane 1 : 293T cell lysates, untreatedLane 2 : 293T cell lysates, treated with EGFLysates/proteins at 10 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/29651_SHC-Primary-antibodies-ab109455-1.gif)
All lanes : Anti-SHC (phospho Y239 + Y240) antibody [EP419] (ab109455) at 1/500 dilutionLane 1 : 293T cell lysates, untreatedLane 2 : 293T cell lysates, treated with EGFLysates/proteins at 10 µg per lane.