Western blot analysis of phosphorylated HSF1 expression in MCF7 whole cell lysates (A,B). Blots were probed with p-HSF1 (Ser 303): sc-101698 preincubated with its cognate phosphorylated peptide (A) and p-HSF1 (Ser 303): sc-101698 (B).
p-HSF1 (Ser 303): sc-101698. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization.
p-HSF1 (Ser 303): sc-101698. Western blot analysis of HSF1 phosphorylation in non-transfected 293T: sc-117752 (A), human HSF1 transfected 293T: sc-171930 (B) and HeLa (C) whole cell lysates.
Western blot analysis of HSF1 phosphorylation in untreated (A, C) and lambda protein phosphatase treated (B, D) HeLa whole cell lysates. Antibodies tested include p-HSF1 (Ser 303): sc-101698 (A, B) and HSF1 (H-311): sc-9144 (C, D).