Anti-Smoothened antibody

• Supplier: Abcam
• Catalog: ab72130
• Prices: $400.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB IHC-P ICC/IF ICC/IF FC
• Species Reactivities: Mouse, Human, Monkey, Zebrafish
• Antigen: Synthetic peptide within Human Smoothened aa 190-240
• Description: Rabbit Polyclonal
• Gene: SMO
• Conjugate: Unconjugated

Product images

All lanes : Anti-Smoothened antibody (ab72130) at 0.1 µg/mlLane 1 : Human heart lysate Lane 2 : Human heart lysate with immunising peptide

Immunohistochemical analysis using formalin/PFA-fixed paraffin-embedded human brain tissue with an isotype control (top image) and ab72130, at 10µg/ml staining Smoothened (bottom image).

ICC/IF image of ab72130 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72130, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ab72130 staining Smoothened in 6 month-old transgenic zebrafish pancreas (Ihha overexpression) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Sections were incubated with primary antibody (1/200) and HRP-conjugated secondary antibody colored using DAB solution. Slides were counterstained with hematoxylin.

Product References

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