Anti-SNAP23 antibody

• Supplier: Abcam
• Catalog: ab3340
• Prices: $398.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ICC/IF ICC/IF IHC-P IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to Mouse SNAP23 aa 193-210
• Description: Rabbit Polyclonal
• Gene: SNAP23
• Conjugate: Unconjugated

Product images

Western blot detection of SNAP23 on rat brain protein extract using ab3340.

ab3340 (1ug/ml) staining SNAP23 in human tonsil using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cellular membrane compartments of the lymphatic nodules.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

ICC/IF image of ab3340 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3340, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

developed using the ECL techniquePerformed under reducing conditions.Exposure time : 2 minutes

Product References

SNARE-dependent interaction of Src, EGFR and beta1 integrin regulates invadopodia - SNARE-dependent interaction of Src, EGFR and beta1 integrin regulates invadopodia

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SNAP23, Syntaxin4, and vesicle-associated membrane protein 7 (VAMP7) mediate - SNAP23, Syntaxin4, and vesicle-associated membrane protein 7 (VAMP7) mediate

Williams KC, McNeilly RE, Coppolino MG. Mol Biol Cell. 2014 Jul 1;25(13):2061-70.

Isoproterenol stimulates transient SNAP23-VAMP2 interaction in rat parotid - Isoproterenol stimulates transient SNAP23-VAMP2 interaction in rat parotid

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Plin2 inhibits cellular glucose uptake through interactions with SNAP23, a SNARE - Plin2 inhibits cellular glucose uptake through interactions with SNAP23, a SNARE

Senthivinayagam S, McIntosh AL, Moon KC, Atshaves BP. PLoS One. 2013 Sep 6;8(9):e73696.

Abnormal myocardial insulin signalling in type 2 diabetes and left-ventricular - Abnormal myocardial insulin signalling in type 2 diabetes and left-ventricular

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Inhibitors of the V0 subunit of the vacuolar H+-ATPase prevent segregation of - Inhibitors of the V0 subunit of the vacuolar H+-ATPase prevent segregation of

Sobota JA, Back N, Eipper BA, Mains RE. J Cell Sci. 2009 Oct 1;122(Pt 19):3542-53.

SNARE proteins mediate fusion between cytosolic lipid droplets and are implicated - SNARE proteins mediate fusion between cytosolic lipid droplets and are implicated

Bostrom P, Andersson L, Rutberg M, Perman J, Lidberg U, Johansson BR, Fernandez-Rodriguez J, Ericson J, Nilsson T, Boren J, Olofsson SO. Nat Cell Biol. 2007 Nov;9(11):1286-93. Epub 2007 Oct 7.