Anti-TATA binding protein TBP antibody [1TB18] - ChIP Grade
| Name | Anti-TATA binding protein TBP antibody [1TB18] - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab12089 |
| Prices | $378.00 |
| Sizes | 50 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 1TB18 |
| Applications | ChIP WB |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein |
| Description | Mouse Monoclonal |
| Gene | TBP |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 8µg of ab12089 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes are located either in the core promoter of the gene (TATA) or in the open reading frame (ORF).
Product References
Efficient double fragmentation ChIP-seq provides nucleotide resolution - Efficient double fragmentation ChIP-seq provides nucleotide resolution
Mokry M, Hatzis P, de Bruijn E, Koster J, Versteeg R, Schuijers J, van de Wetering M, Guryev V, Clevers H, Cuppen E. PLoS One. 2010 Nov 30;5(11):e15092.
Increased c-Jun expression and reduced GATA2 expression promote aberrant - Increased c-Jun expression and reduced GATA2 expression promote aberrant
Yang Z, Kondo T, Voorhorst CS, Nabinger SC, Ndong L, Yin F, Chan EM, Yu M, Wurstlin O, Kratz CP, Niemeyer CM, Flotho C, Hashino E, Chan RJ. Mol Cell Biol. 2009 Aug;29(16):4376-93.