Anti-TCP1 beta antibody [EPR4084]
| Name | Anti-TCP1 beta antibody [EPR4084] |
|---|---|
| Supplier | Abcam |
| Catalog | ab92746 |
| Prices | $387.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPR4084 |
| Applications | WB IP IHC-P FC ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to residues on the C terminus of Human TCP1 beta |
| Description | Rabbit Monoclonal |
| Gene | CCT2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-TCP1 beta antibody [EPR4084] (ab92746) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : MCF-7 cell lysateLane 3 : Daudi cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/9352_TCP1-beta-Primary-antibodies-ab92746-1.jpg)
All lanes : Anti-TCP1 beta antibody [EPR4084] (ab92746) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : MCF-7 cell lysateLane 3 : Daudi cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
ab92746, at a 1/100 dilution, staining TCP1 beta in formalin fixed, paraffin embedded (1) Human colon tissue and (2) Human kidney tissue by Immunohistochemistry. Detection: DAB staining.
ab92746, at a 1/100 dilution, staining TCP1 beta in HeLa cells by Immunofluorescence.
Overlay histogram showing HeLa cells stained with ab92746 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92746, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Modulation of STAT3 folding and function by TRiC/CCT chaperonin. - Modulation of STAT3 folding and function by TRiC/CCT chaperonin.
Kasembeli M, Lau WC, Roh SH, Eckols TK, Frydman J, Chiu W, Tweardy DJ. PLoS Biol. 2014 Apr 22;12(4):e1001844.
TCP1 complex proteins interact with phosphorothioate oligonucleotides and can - TCP1 complex proteins interact with phosphorothioate oligonucleotides and can
Liang XH, Shen W, Sun H, Prakash TP, Crooke ST. Nucleic Acids Res. 2014 Jul;42(12):7819-32.