Anti-TFIIE alpha antibody - ChIP Grade
| Name | Anti-TFIIE alpha antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab28177 |
| Prices | $370.00 |
| Sizes | 250 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ChIP |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein (Human): TFIIE alpha |
| Description | Rabbit Polyclonal |
| Gene | GTF2E1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 8µg of ab28177 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes are located either in the core promoter of the gene (TATA) or in the open reading frame (ORF).