Anti-TGF alpha antibody [P/T1]
| Name | Anti-TGF alpha antibody [P/T1] |
|---|---|
| Supplier | Abcam |
| Catalog | ab9578 |
| Prices | $379.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | P/T1 |
| Applications | ELISA IHC-P IHC-F ICC/IF FC |
| Species Reactivities | Human |
| Antigen | Synthetic peptide fragment (32-40 amino acids sequence of 50 amino acids) of transforming growth factor alpha (TGF-alpha) |
| Description | Mouse Monoclonal |
| Gene | TGFA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemical characterization of the anti-TGF-α monoclonal antibody ab9578 on human melanoma.
![Overlay histogram showing A431 cells stained with ab9578 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9578, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/10232_TGF-alpha-Primary-antibodies-ab9578-1.jpg)
Overlay histogram showing A431 cells stained with ab9578 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9578, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Product References
Both stromal cell and colonocyte epidermal growth factor receptors control HCT116 - Both stromal cell and colonocyte epidermal growth factor receptors control HCT116
Mustafi R, Dougherty U, Shah H, Dehghan H, Gliksberg A, Wu J, Zhu H, Joseph L, Hart J, Dive C, Fichera A, Threadgill D, Bissonnette M. Carcinogenesis. 2012 Oct;33(10):1930-9.
[Creation of monoclonal antibodies against tumor necrosis factor-alpha - [Creation of monoclonal antibodies against tumor necrosis factor-alpha
Bebok Z, Szekeres G, Horvath G, Duda E, Nemeth P. Orv Hetil. 1993 Jun 13;134(24):1303-7.