Anti-TIF1 alpha antibody
| Name | Anti-TIF1 alpha antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab70560 |
| Prices | $370.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB IP |
| Species Reactivities | Mouse, Human, Rat, Rabbit, Horse, Guinea Pig, Bovine, Dog, Chimpanzee, Monkey, Ape, Hamster, Orangutan |
| Antigen | Synthetic peptide corresponding to a region between residue 700 and 750 of human TIF1 alpha (NP_056989 |
| Description | Rabbit Polyclonal |
| Gene | TRIM24 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling TIF1 alpha with ab70560 at 1/1000 (0.2µg/ml). Detection: DAB.
IHC image of TIF1 alpha staining in Human colon adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70560, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-TIF1 alpha antibody (ab70560) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgLane 5 : NIH3T3whole cell lysate at 50 µg