Anti-TMEM16A antibody
| Name | Anti-TMEM16A antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab84915 |
| Prices | $376.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human TMEM16A |
| Description | Rabbit Polyclonal |
| Gene | ANO1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-TMEM16A antibody (ab84915) at 1 µg/ml + Brain (Human) Membrane Lysate - adult normal tissue (ab29456) at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab84915 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84915, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.