Anti-TNF alpha Affibody® Molecule (Agarose)
| Name | Anti-TNF alpha Affibody® Molecule (Agarose) |
|---|---|
| Supplier | Abcam |
| Catalog | ab31909 |
| Prices | $698.00 |
| Sizes | 200 µl |
| Applications | IP |
| Species Reactivities | Human |
| Antigen | - |
| Gene | TNF |
| Conjugate | Agarose |
| Supplier Page | Shop |
Product images
The agarose immobilized Anti-TNF alpha Affibody® molecule precipitates TNF-alpha protein from cell extracts derived from LPS stimulated but not from unstimulated THP-1 cells. RESULTS IMMUNOPRECIPITATION The human pro-monocytic cell line THP-1 was primed with PMA (200 nM) over night and stimulated with LPS (200 ng/ml) or with PBS (control) for 2 hours prior to termination of culture. Cell extracts prepared from LPS stimulated cultures and from control cells were incubated with agarose immobilized Anti-TNF-alpha Affibody® molecule for 2 hours. After incubation, the unbound proteins were washed away and the bound protein was eluted with SDS-PAGE separation and blotted onto a PVDF filter. The filter was stained with an antibody against full length TNF-alpha protein (25 kD). Figure 1a and 1b show that TNF-alpha was precipitated from LPS stimulated, but not from PBS stimulated THP-1 cell extract, using agarose immobilized Anti-TNF alpha Affibody® molecule. With increased amount of total prot