Standard transferrin was titrated on Anti-Transferrin Affibody® molecule coated plates with a sensitivity of 30 ng/ml. QUANTITATIVE ELISA The Anti-Transferrin Affibody® molecule can be used as capture reagent in a sandwich ELISA in combination with a rabbit anti-transferrin antibody as the detection reagent. Titration of transferrin gives a sigmoid curve with a sensitivity of 30 ng transferrin/ml (defined as two times background value) and a measurement interval between 100 and 1000 ng/ml.
ANALYSIS OF TRANSFERRIN CONCENTRATION IN DEPLETED PLASMA The concentration of transferrin in plasma samples previously depleted from transferrin by passage through an Anti-Transferrin Affibody® molecule SulfoLink® Coupling Gel column, was analyzed using the Anti-Transferrin Affibody® ELISA. Flow through samples from 60, 600, 800 and 1200 ul depleted plasma were analyzed, the concentration of transferrin in the samples and the percentage of achived depletion are presented in table 1.