Anti-Transferrin antibody

• Supplier: Abcam
• Catalog: ab82411
• Prices: $390.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-P ICC/IF ICC/IF WB IP
• Species Reactivities: Mouse, Rat, Guinea Pig, Dog, Human
• Antigen: Human Transferrin Protein
• Description: Rabbit Polyclonal
• Gene: TF
• Conjugate: Unconjugated

Product images

Anti-Transferrin antibody (ab82411) at 1/10000 dilution + rat apotransferrindeveloped using the ECL technique

ICC/IF image of ab82411 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82411, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

IHC image of Transferrin staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82411, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

PHEX mimetic (SPR4-peptide) corrects and improves HYP and wild type mice - PHEX mimetic (SPR4-peptide) corrects and improves HYP and wild type mice

Zelenchuk LV, Hedge AM, Rowe PS. PLoS One. 2014 May 19;9(5):e97326.

Dynamic changes of urinary proteins in a focal segmental glomerulosclerosis rat - Dynamic changes of urinary proteins in a focal segmental glomerulosclerosis rat

Zhao M, Li M, Li X, Shao C, Yin J, Gao Y. Proteome Sci. 2014 Jul 21;12:42.

Transgenic mice expressing an intracellular fluorescent fusion of angiotensin II - Transgenic mice expressing an intracellular fluorescent fusion of angiotensin II

Redding KM, Chen BL, Singh A, Re RN, Navar LG, Seth DM, Sigmund CD, Tang WW, Cook JL. Am J Physiol Heart Circ Physiol. 2010 Jun;298(6):H1807-18. doi: