Anti-Transferrin antibody
| Name | Anti-Transferrin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab9538 |
| Prices | $378.00 |
| Sizes | 1 ml |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA ICC/IF ICC/IF IHC-P IHC-F |
| Species Reactivities | Human |
| Antigen | Transferrin isolated from human serum |
| Description | Rabbit Polyclonal |
| Gene | TF |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Standard curve for Transferrin (Analyte: ab83560); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [HTF-14] to Transferrin (ab769) at 1µg/ml and Detector Antibody Rabbit polyclonal to Transferrin (ab9538) at 0.5µg/ml.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/13893_Transferrin-Primary-antibodies-ab9538-5.jpg)
Standard curve for Transferrin (Analyte: ab83560); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [HTF-14] to Transferrin (ab769) at 1µg/ml and Detector Antibody Rabbit polyclonal to Transferrin (ab9538) at 0.5µg/ml.
ICC/IF image of ab9538 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9538, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Ab9538 staining transferrin in human liver parenchima. Staining is localised to intracellular compartment and extracellular space.Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification