All lanes : Anti-TUBA3E antibody [2B4] (ab124019) at 1/2000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control Lane 2 : HEK293T cells transfected with pCMV6-ENTRY TUBA3E Lysates/proteins at 5 µg per lane.
ab124019, at 1/100, staining TUBA3E in COS7 cells transiently transfected by pCMV6-ENTRY TUBA3E by Immunofluorescence.
HEK293T cells transfected with either TUBA3E overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab124019, at 1/100, and then analyzed by Flow cytometry.