Anti-Tyrosinase antibody - C-terminal (ab175997) at 1/100 dilution + HepG2 cell line lysate at 35 µg
Immunohistochemical analysis of paraffin embedded Human skin tissue labeling Tyrosinase with ab175997 at 1/10 followed by peroxidase conjugation of the secondary antibody and DAB staining.
Immunofluorescent analysis of HepG2 cells labeling Tyrosinase with ab175997 at 1/10 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Flow Cytometrical analysis of A375 cells labeling Tyrosinase with ab175997 at 1/10 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.