Anti-UGT2B17 antibody (ab92610) at 1/50 dilution + Y79 cell line lysates at 35 µg
ICC/IF image of ab92610 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92610, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab92610 staining UGT2B17 in LNCaP Androgen Dependent Prostate Cancer cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in Triton X-100 prior to blocking in 5% serum for 1 hour at 20°C. The primary antibody was diluted 1/50 in PBS/1%BSA and incubated with the sample for 24 hours at 4°C. The secondary antibody was Alexa Fluor® 488-conjugated goat anti-rabbit polyclonal, diluted 1/250. Blue nuclear counterstain is Hoechst staining.See Abreview