Anti-Wnt2 antibody
| Name | Anti-Wnt2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab27794 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide (Human) -surrounding amino acid 45 of Wnt2 |
| Description | Rabbit Polyclonal |
| Gene | WNT2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Wnt2 antibody (ab27794) at 2 µg/ml + Rat kidney tissue lysate. at 30 µg
ICC/IF image of ab27794 stained MCF7 cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27794, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab27794 staining Wnt2 in murine coronal tissue by Immunohistochemistry (PFA perfusion fixed frozen sections).(i) Developing aorta.(ii) and (iii) Medio-lateral regions of the developing mesencephalon.Abbreviations: BC, blood cells; AA, aorta; VM, ventral midbrain.
Product References
Heparan sulfate regulates hair follicle and sebaceous gland morphogenesis and - Heparan sulfate regulates hair follicle and sebaceous gland morphogenesis and
Coulson-Thomas VJ, Gesteira TF, Esko J, Kao W. J Biol Chem. 2014 Sep 5;289(36):25211-26.
Wnt2 regulates progenitor proliferation in the developing ventral midbrain. - Wnt2 regulates progenitor proliferation in the developing ventral midbrain.
Sousa KM, Villaescusa JC, Cajanek L, Ondr JK, Castelo-Branco G, Hofstra W, Bryja V, Palmberg C, Bergman T, Wainwright B, Lang RA, Arenas E. J Biol Chem. 2010 Mar 5;285(10):7246-53.