Anti-XIAP antibody
| Name | Anti-XIAP antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab2541 |
| Prices | $390.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Available on request |
| Blocking Peptide | XIAP peptide |
| Description | Rabbit Polyclonal |
| Gene | XIAP |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab2541 (1/200) staining XIAP in HeLa cells (green). Cells were permeabilised with 0.5% Triton-X100/ PBS, fixed in methanol (-20oC, 10min) and counterstained with DAPI (red) in order to highlight the nucleus. Please refer to abreview for further experimental details.See Abreview
All lanes : Anti-XIAP antibody (ab2541) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab2541 staining XIAP in MCF7 cells treated with forskolin (ab120058), by ICC/IF. Increase in XIAP expression correlates with increased concentration of forskolin, as described in literature.The cells were incubated at 37°C for 2h in media containing different concentrations of ab120058 (forskolin) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab2541 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
Product References
Anti-apoptotic effects of osteopontin through the up-regulation of Mcl-1 in - Anti-apoptotic effects of osteopontin through the up-regulation of Mcl-1 in
Hsu KH, Tsai HW, Lin PW, Hsu YS, Lu PJ, Shan YS. World J Surg Oncol. 2014 Jun 20;12:189.
Apollon gene silencing induces apoptosis in breast cancer cells through p53 - Apollon gene silencing induces apoptosis in breast cancer cells through p53
Lopergolo A, Pennati M, Gandellini P, Orlotti NI, Poma P, Daidone MG, Folini M, Zaffaroni N. Br J Cancer. 2009 Mar 10;100(5):739-46.
Silencing of X-linked inhibitor of apoptosis (XIAP) decreases gemcitabine - Silencing of X-linked inhibitor of apoptosis (XIAP) decreases gemcitabine
Shrikhande SV, Kleeff J, Kayed H, Keleg S, Reiser C, Giese T, Buchler MW, Esposito I, Friess H. Anticancer Res. 2006 Sep-Oct;26(5A):3265-73.