Anti-Zyxin antibody
| Name | Anti-Zyxin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab71842 |
| Prices | $394.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP ICC/IF ICC/IF WB |
| Species Reactivities | Rat, Human, Mouse, Dog, Monkey |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human Zyxin |
| Description | Rabbit Polyclonal |
| Gene | ZYX |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Zyxin antibody (ab71842) at 1 µg/mlLane 1 : SK N SH (Human neuroblastoma) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 3 : A498 (Human Kidney Carcinoma) Whole Cell LysateLane 4 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell LysateLane 5 : DU 145 (Human prostate carcinoma cell line) Whole Cell LysateLane 6 : Testis (Rat) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
![Zyxin was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Zyxin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab71842.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 75kDa; Zyxin](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/25175_Zyxin-Primary-antibodies-ab71842-13.jpg)
Zyxin was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Zyxin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab71842.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 75kDa; Zyxin
ICC/IF image of ab71842 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71842, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa cells at 5µg/ml, and in 100% Methanol fixed (5 min) Hela, Hek293, HepG2, and MCF-7 cells at 5µg/ml.
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