NFκB p50 (E-10)

Name	NFκB p50 (E-10)
Supplier	Santa Cruz Biotechnology
Catalog	sc-8414
Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal
Isotype	mouse IgG 1
Applications	WB IP ICC/IF IHC-P FC ELISA
Species Reactivities	Mouse, Rat, Human
Antigen	120-239 (h)
Description	Mouse Monoclonal
Gene	NFKB1
Supplier Page	Shop

Product images

NFκB p50 (E-10): sc-8414. Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear localization using indirect FITC (A) staining and HeLa cells showing cytoplasmic localization using direct Alexa Fluor 488 (B) staining.

NFκB p50 (E-10) Alexa Fluor 647: sc-8414 AF647. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG₁: sc-24636.

NFκB p50 (E-10) Alexa Fluor 488: sc-8414 AF488. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG₁: sc-3890.

Western blot analysis of NFκB p50 phosphorylation in untreated (A,D), TNFα and calyculin A treated (B,E) and TNFα, calyculin A and lambda protein phosphatase treated (C,F) HeLa whole cell lysates. Antibodies tested include p-NFκB p50 (A-8): sc-271908 (A,B,C) and NFκB p50 (E-10): sc-8414 (D,E,F).

ChIP analysis of cofactor occupancy dynamics on the <i>IL</i>-<i>8</i> promoter in 293 cells in response to IL-1β treatment. Antibodies tested include NFκB p50 (C-19): sc-1190, NFκB p50 (E-10): sc-8414, NFκB p50 (H-119): sc-7178, NFκB p65 (C-20): sc-372, NFκB p65 (A): sc-109, NFκB p65 (H-286): sc-7151, CBP (A-22): sc-369, CBP (C-1): sc-7300, CBP (C-20): sc-583, CBP (451): sc-1211, p300 (C-20): sc-sc-585, p300 (N-15): sc-584, p300 (H-272): sc-8981. Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek <i>et al</i>., Cell 2002, 110: 55-67.

ChIP analysis of cofactor occupancy dynamics on the IL-8 promoter in 293 cells in response to IL-1β treatment. Antibodies tested include NFκB p50 (C-19): sc-1190, NFκB p50 (E-10): sc-8414, NFκB p50 (H-119): sc-7178, NFκB p65 (C-20): sc-372, NFκB p65 (A): sc-109, NFκB p65 (H-286): sc-7151, CBP (A-22): sc-369, CBP (C-1): sc-7300, CBP (C-20): sc-583, CBP (451): sc-1211, p300 (C-20): sc-sc-585, p300 (N-15): sc-584, p300 (H-272): sc-8981. Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.

ChIP analysis of cofactor occupancy dynamics on the <i>ICAM1</i> promoter in 293 cells in response to IL-1β treatment. Antibodies tested include NFκB p50 (C-19): sc-1190, NFκB p50 (E-10): sc-8414, NFκB p50 (H-119): sc-7178, NFκB p65 (C-20): sc-372, NFκB p65 (A): sc-109, NFκB p65 (H-286): sc-7151, Bcl-3 (C-14): sc-185, Bcl-3 (H-146): sc-13038, PCAF (C-16): sc-6300, PCAF (H-369): sc-8999, CBP (A-22): sc-369, CBP (C-1): sc-7300, CBP (C-20): sc-583, CBP (451): sc-1211. Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek <i>et al</i>., Cell 2002, 110: 55-67.

ChIP analysis of cofactor occupancy dynamics on the ICAM1 promoter in 293 cells in response to IL-1β treatment. Antibodies tested include NFκB p50 (C-19): sc-1190, NFκB p50 (E-10): sc-8414, NFκB p50 (H-119): sc-7178, NFκB p65 (C-20): sc-372, NFκB p65 (A): sc-109, NFκB p65 (H-286): sc-7151, Bcl-3 (C-14): sc-185, Bcl-3 (H-146): sc-13038, PCAF (C-16): sc-6300, PCAF (H-369): sc-8999, CBP (A-22): sc-369, CBP (C-1): sc-7300, CBP (C-20): sc-583, CBP (451): sc-1211. Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.

ChIP analysis of cofactor occupancy dynamics on the <i>KAI1</i> promoter in 293 cells in response to IL-1β treatment. Antibodies tested include NFκB p50 (C-19): sc-1190, NFκB p50 (E-10): sc-8414, NFκB p50 (H-119): sc-7178, NFκB p65 (C-20): sc-372, NFκB p65 (A): sc-109, NFκB p65 (H-286): sc-7151, HDAC3 (H-99): sc-11417, HDAC3 (N-19): sc-8138, Bcl-3 (C-14): sc-185, Bcl-3 (H-146): sc-13038. Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek <i>et al</i>., Cell 2002, 110: 55-67.

ChIP analysis of cofactor occupancy dynamics on the KAI1 promoter in 293 cells in response to IL-1β treatment. Antibodies tested include NFκB p50 (C-19): sc-1190, NFκB p50 (E-10): sc-8414, NFκB p50 (H-119): sc-7178, NFκB p65 (C-20): sc-372, NFκB p65 (A): sc-109, NFκB p65 (H-286): sc-7151, HDAC3 (H-99): sc-11417, HDAC3 (N-19): sc-8138, Bcl-3 (C-14): sc-185, Bcl-3 (H-146): sc-13038. Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.

NFκB p50 (E-10) PE: sc-8414 PE. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG₁: sc-2866.

NFκB p50 (E-10): sc-8414. Western blot analysis of NFκB p50 and p105 expression in K-562 whole cell lysate.

NFκB p50 (E-10): sc-8414. Immunofluroescence staining of methanol-fixed K-562 cells showing nuclear staining.

NFκB p50 (E-10): sc-8414. Western blot analysis of NFκB p50 expression in non-transfected: sc-117752 (A) and human NFκB p50 transfected: sc-116112 (B) 293T whole cell lysates.

NFκB p50 (E-10): sc-8414. Western blot analysis of NFκB p50 expression in non-transfected: sc-117752 (A) and mouse NFκB p50 transfected: sc-122025 (B) 293T whole cell lysates.

NFκB p50 (E-10): sc-8414. Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing cytoplasmic staining of glandular cells.

Western blot analysis of NFκB p50 phosphorylation in untreated (A,D), TNF and calyculin A treated (B,E) and TNF, calyculin A and lambda protein phosphatase treated (C,F) HeLa whole cell lysates. Antibodies tested include p-NFκB p50 (Ser 932): sc-101747 (A,B,C) and NFκB p50 (E-10): sc-8414 (D,E,F).

Western blot analysis of NFκB p50 phosphorylation in untreated (A,D), calyculin A treated (B,E) and calyculin A and lambda protein phosphatase treated (C,F) HeLa whole cell lysates. Antibodies tested include p-NFκB p50 (Ser 932): sc-101747 (A,B,C) and NFκB p50 (E-10): sc-8414 (D,E,F).