Anti-ACO2 / Aconitase 2 Antibody (aa438-467) IHC-plusâ¢
| Name | Anti-ACO2 / Aconitase 2 Antibody (aa438-467) IHC-plus⢠|
|---|---|
| Supplier | LifeSpan Bioscience |
| Catalog | LS-B3427 |
| Prices | $395.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Applications | IHC-P WB |
| Species Reactivities | Human, Mouse, Rat |
| Purity/Format | Ammonium sulfate precipitation |
| Blocking Peptide | ACLP / AEBP1 Antibody Blocking Peptide |
| Description | Rabbit Polyclonal |
| Gene | ACO2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-ACO2 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3427 concentration 10 ug/ml.
Anti-ACO2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3427 concentration 10 ug/ml.
ACO2 western blot of 293 cell line lysates (35 ug/lane). The Aconitase antibody detected the Aconitase protein (arrow).
![Perfused isolated rat heart whole tissue lysate was lysed with either A) 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% Na-deoxycholate, 1 mM Na3VO4, 20 mM NaF, 1 mM PMSF, 5 v/v % protease inhibitor cocktail or B) T-PER Tissue Protein Extraction Reagent [# 785101; Pierce], containing 1mM Na3VO4, 20 mM NaF, 5 v/v % protease inhibitor cocktail (Sigma); PVDF membrane was incubated in primary Ab [rabbit polyclonal antibody against ACO2. Solution: 1:1000 diluted in 5% NFM TBS-T 0,05 for overnight (15 hrs) at 4 ?. Data courtesy of Boglarka Laczy M.D., Division of Cardiovascular Disease, Dept. of Medicine, University of Alabama at Birmingham.](http://www.bioprodhub.com/system/product_images/ab_products/3/sub_1/1384_225014_1611079.jpg)
Perfused isolated rat heart whole tissue lysate was lysed with either A) 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% Na-deoxycholate, 1 mM Na3VO4, 20 mM NaF, 1 mM PMSF, 5 v/v % protease inhibitor cocktail or B) T-PER Tissue Protein Extraction Reagent [# 785101; Pierce], containing 1mM Na3VO4, 20 mM NaF, 5 v/v % protease inhibitor cocktail (Sigma); PVDF membrane was incubated in primary Ab [rabbit polyclonal antibody against ACO2. Solution: 1:1000 diluted in 5% NFM TBS-T 0,05 for overnight (15 hrs) at 4 ?. Data courtesy of Boglarka Laczy M.D., Division of Cardiovascular Disease, Dept. of Medicine, University of Alabama at Birmingham.
Western blot of anti-ACO2 antibody in mouse heart(left) and 293 (right) tissue lysates (35 ug/lane). ACO2(arrow) was detected using the purified antibody.