TRX2 Antibody

• Supplier: Bethyl Labs
• Catalog: A300-113A
• Prices: $339.00
• Sizes: 100 µl (1 mg/ml)
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB IP ChIP-PCR ChIP-on-chip PLA
• Species Reactivities: Human
• Antigen: Between 2375 and 2425
• Purity/Format: Whole IgG
• Description: Rabbit Polyclonal
• Gene: KMT2B

Product images

Detection of Human Trithorax 2 by Western Blot and Immunoprecipitation.  Samples: A. Whole cell lysate (100 µg - E; 10 µg - T) from 293T cells that were untreated (E) or transfected with an expression construct containing the residues c-terminal to the consensus cleavage site within TRX2 (T).  B.  Whole cell lysate (100 µg - Input; one 10 cm plate for IP) from untreated 293T cells.  Antibodies:  Affinity purified rabbit anti-TRX2 antibody A300-113A used at the indicated concentrations for WB (A) and 10 µg/plate for IP (B).   Immunoprecipitated TRX2 was detected by WB using A300-113A or rabbit anti-TRX2 antibody BL836.  Detection: Chemiluminescence with exposure times of 10 (A) or 1 seconds (B).
  
  
  

ChIP-chip scatter plot of anti-TRX2 (A300-113A) enriched DNA binding sites versus input reference DNA. A. 10 µg of A300-113A was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-TRX2 ChIP, normal rabbit IgG showed little enrichment.
  
  
  

This antibody is qualified for the Proximity Ligation Assay (PLA). PLA uses paired antibodies, so please refer to the PLA page of our website for specific imaging obtained by pairing this antibody with other qualified antibodies. The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
  
  
  

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