beta-3 Tubulin Monoclonal Antibody (TU-20)
| Name | beta-3 Tubulin Monoclonal Antibody (TU-20) |
|---|---|
| Supplier | Thermo Fisher Scientific |
| Catalog | MA1-19187 |
| Prices | $285.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | TU-20 |
| Applications | FC ICC/IF IHC-P WB |
| Antigen | Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide |
| Purity/Format | Ammonium sulfate precipitation, Caprylic acid precipitation |
| Description | Mouse Monoclonal |
| Gene | TUBB3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
beta-3 Tubulin Antibody (MA1-19187)
Immunofluorescence analysis of Beta-3 Tubulin was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Beta-3 Tubulin (TU-20) Mouse Monoclonal Antibody (MA119187) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.