Anti-AP1G1 / Gamma Adaptin antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B344 concentration 5 ug/ml.
Anti-AP1G1 Antibody - Western Blot. Western blot of Affinity Purified anti-AP1G1 antibody shows strong detection of a 91-kD band corresponding to Human AP1G1 in a HeLa whole cell lysate (lane 1). Peptide competition (using 1 ug/ml of the immunizing peptide) blocks the specific reactivity of this antibody with AP1G1 (lane 2). Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP1G1 antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemi-luminescence system was used for signal detection (Roche) using a 60-sec exposure time.