Formalin-fixed and paraffin-embedded human brain with APP Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of APP (arrow) using rabbit polyclonal APP Antibody (RB05012). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the APP gene (Lane 2) (Origene Technologies).
Flow cytometric of HepG2 cells using APP Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.