BMP-6 in Mouse Embryo. BMP-6 was detected in immersion fixed frozen sections of mouse embryo (15 d. p. c.) using Sheep Anti-Mouse BMP-6 Antigen Affinity-purified Polyclonal Antibody at 15 ug/ml overnight at 4°C. Tissue was stained using Anti-Sheep HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to skeletal muscle cells.
Detection of Mouse BMP-6 by Western Blot. Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with mouse BMP-6. PVDF membrane was probed with 1 ug/ml of Sheep Anti-Mouse BMP-6 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. For additional reference, Recombinant Mouse BMP-6 (10 ng/lane) was included. A specific band was detected for BMP-6 at approximately 20kD (as indicated). This experiment was conducted under reducing conditions.