RIPAb+ Upf1 - RIP Validated Antibody and Primer Set

Name	RIPAb+ Upf1 - RIP Validated Antibody and Primer Set
Supplier	EMD Millipore
Catalog	03-191
Prices	$389.00
Sizes	10 assays 10 assays per set. recommended use: ~7 μl of antibody per rip . (dependent upon biological context)
Host	Rabbit
Clonality	Polyclonal
Applications	IP RIP-Chip WB
Species Reactivities	Dog, Bovine, Human, Mouse, Rat
Antigen	KLH-conjugated synthetic linear peptide corresponding to Upf1.
Purity/Format	Affinity Purfied
Description	Rabbit Polyclonal
Gene	UPF1
Supplier Page	Shop

Product images

Immunoprecipitation from RIP lysate: <br />Representative lot data. <br />RIP lysate from HeLa cells (~2 X 10E7 cell equivalents per IP) was subjected to immunoprecipitation using either 7 µL of a normal rabbit IgG or 7 µL of Anti-Upf1 antibody. <br />Ten percent of the precipitated proteins (lane 1: rabbit IgG, lane 2: Upf1) were resolved by electrophoresis, transferred to PVDF and probed with anti-Upf1 antibody (1:2000). <br />Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00232) <br />Arrow indicates Upf1 (~125 kDa) (Figure 2).

Immunoprecipitation from RIP lysate:
Representative lot data.
RIP lysate from HeLa cells (~2 X 10E7 cell equivalents per IP) was subjected to immunoprecipitation using either 7 µL of a normal rabbit IgG or 7 µL of Anti-Upf1 antibody.
Ten percent of the precipitated proteins (lane 1: rabbit IgG, lane 2: Upf1) were resolved by electrophoresis, transferred to PVDF and probed with anti-Upf1 antibody (1:2000).
Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00232)
Arrow indicates Upf1 (~125 kDa) (Figure 2).