RIPAb+ EF1α - RIP Validated Antibody and Primer Set

Name	RIPAb+ EF1α - RIP Validated Antibody and Primer Set
Supplier	EMD Millipore
Catalog	03-107
Prices	$409.00
Sizes	10 assays 10 assays per set. recommended use: ~5 μg of antibody per rip . (dependent upon biological context)
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1κ
Applications	WB RIP-Chip IP
Species Reactivities	Yeast, Human, Mammalian
Antigen	Crude calmodulin-binding proteins from Trypanosoma brucei isolated using calmodulin-affinity chromatography.
Description	Mouse Monoclonal
Gene	EEF1A1
Supplier Page	Shop

Product images

Immunoprecipitation from RIP Lysate:<br />Representative lot data.<br />RIP lysate from HeLa cells (2 X 106 cell equivalents per IP) was subjected to immunoprecipitation using 0.5 µg of either a normal mouse IgG, (Part # CS200621), or Anti-EF1α antibody (Part # CS204384). Precipitated proteins (Lane 1: mouse IgG, Lane 2: anti-EF1α) and HeLa whole cell lysate (Lane 3) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-EF1α antibody (Part # CS204384, 1.0 µg/mL). <br />Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00232). <br /><br />Arrow indicates EF1α.<br />

Immunoprecipitation from RIP Lysate:
Representative lot data.
RIP lysate from HeLa cells (2 X 106 cell equivalents per IP) was subjected to immunoprecipitation using 0.5 µg of either a normal mouse IgG, (Part # CS200621), or Anti-EF1α antibody (Part # CS204384). Precipitated proteins (Lane 1: mouse IgG, Lane 2: anti-EF1α) and HeLa whole cell lysate (Lane 3) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-EF1α antibody (Part # CS204384, 1.0 µg/mL).
Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00232).

Arrow indicates EF1α.