Western blot analysis of recombinant human CTRPs using anti-CTRP5 (human), pAb at 1:4,000 dilution.1: Recombinant human CTRP5 protein (His-tagged).2: Unrelated recombinant protein (His-tagged).
Immunoprecipitation (IP) analysis of the cell lysates from HEK293 cells transfected with empty vector or a panel of the FLAG-tagged CTRP family (full-length) followed by immunoblot analysis using anti-CTRP5 (human), pAb at 1:4,000 dilution. The immunoblot (IB) analysis demonstrates the specificity of the anti-CTRP5 (human), pAb antibody. 3: hCTRP1-FLAG (full-length) transfected HEK293 cell lysates. 4: hCTRP2-FLAG (full-length) transfected HEK293 cell lysates. 5: hCTRP3-FLAG (full-length) transfected HEK293 cell lysates. 6: hCTRP5-FLAG (full-length) transfected HEK293 cell lysates.. 7: hCTRP6-FLAG (full-length) transfected HEK293 cell lysates. 8: hCTRP7-FLAG (full-length) transfected HEK293 cell lysates. 9: hCTRP9-FLAG (full-length) transfected HEK293 cell lysates. 10: hCTRP10-FLAG (full-length) transfected HEK293 cell lysates. 11: hCTRP11-FLAG (full-length) transfected HEK293 cell lysates. 12: hCTRP13-FLAG (full-length) transfected HEK293 cell lysates. 13: Empty vector transfected HEK2