
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-CARD8 mAb.

Western blot analysis of immunized recombinant protein, using anti-CARD8 monoclonal antibody.

Detection of human CARD8 by Western blot. Samples: Whole cell lysate (50 ug) from HeLa cells. Predicted molecular weight: 48 kDa

Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-CARD8 mAb. Predicted molecular weight: 48 kDa