
All lanes : Anti-Hmga2 Antibody (C-term) at 1:1000 dilution Lane 1: F9 whole cell lysates Lane 2: HepG2 whole cell lysates Lane 3: NCCIT whole cell lysates Lane 4: NIH/3T3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 12 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent image of C2C12cells stained with Mouse Hmga2 Antibody (C-term)(Cat#WA1383). WA1383 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).