ER alpha monoclonal antibody - Classic

Name	ER alpha monoclonal antibody - Classic
Supplier	Diagenode
Catalog	C15100066
Prices	$295.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Applications	ChIPseq ChIP-PCR WB ELISA IP ICC/IF
Species Reactivities	Human
Purity/Format	Ascites fluid
Description	Mouse Monoclonal
Supplier Page	Shop

Product images

<strong> Figure 1. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against ERalpha </strong><br />ChIP was performed with the Diagenode monoclonal antibody against ERalpha (cat. No. AC-066-100) on sheared chromatin from MCF7 cells treated for 1 hour with estradiol. The IP’d DNA was analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 1 shows the obtained peaks near the TFF1 gene on chromosome 21 (figure 1A), the GREB1 and HAAO genes on chromosome 2 (figure 1B and C), and the ZNF185 gene on the X-chromosome (figure 1D).

Figure 1. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against ERalpha
ChIP was performed with the Diagenode monoclonal antibody against ERalpha (cat. No. AC-066-100) on sheared chromatin from MCF7 cells treated for 1 hour with estradiol. The IP’d DNA was analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 1 shows the obtained peaks near the TFF1 gene on chromosome 21 (figure 1A), the GREB1 and HAAO genes on chromosome 2 (figure 1B and C), and the ZNF185 gene on the X-chromosome (figure 1D).