H3K18ac polyclonal antibody - Classic (sample size)

Name	H3K18ac polyclonal antibody - Classic (sample size)
Supplier	Diagenode
Catalog	C15410139-10
Prices	$80.00
Sizes	10 μg
Host	Rabbit
Clonality	Polyclonal
Applications	ChIPseq ChIP-PCR WB ELISA DB ICC/IF
Species Reactivities	Human, Mouse
Purity/Format	Affinity purified
Description	Rabbit Polyclonal
Supplier Page	Shop

Product images

<strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against H3K18ac</strong><br /> ChIP assays were performed using human HeLa cells, treated with TSA, the Diagenode antibody against H3K18ac (cat. No. C15410139) and optimized PCR primer pairs for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. AB-001-0024), using sheared chromatin from 1,000,000 cells. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active EIF4A2 and c-fos genes, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Figure 1. ChIP results obtained with the Diagenode antibody directed against H3K18ac
ChIP assays were performed using human HeLa cells, treated with TSA, the Diagenode antibody against H3K18ac (cat. No. C15410139) and optimized PCR primer pairs for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. AB-001-0024), using sheared chromatin from 1,000,000 cells. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active EIF4A2 and c-fos genes, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

<strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against H3K18ac</strong><br /> ChIP was performed as described above using 1 μg of the Diagenode antibody against H3K18ac (cat. No. C15410139). The IP’d DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete human X-chromosome and a zoomin to a 600 kb region (figure 2A and B), and in two regions on chromosome 14 and 3 surrounding the c-fos and EIF4A2 positive control genes (figure 2C and D, respectively).

Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against H3K18ac
ChIP was performed as described above using 1 μg of the Diagenode antibody against H3K18ac (cat. No. C15410139). The IP’d DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete human X-chromosome and a zoomin to a 600 kb region (figure 2A and B), and in two regions on chromosome 14 and 3 surrounding the c-fos and EIF4A2 positive control genes (figure 2C and D, respectively).

<strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody against H3K18ac (cat. No. C15410139). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:4,300.

Figure 3. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody against H3K18ac (cat. No. C15410139). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:4,300.

<strong> Figure 4. Cross reactivity tests using the Diagenode antibody directed against H3K18ac</strong><br /> To test the cross reactivity of the Diagenode antibody against H3K18ac (cat. No. C15410139), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K18. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:5,000. Figure 4 shows a high specificity of the antibody for the modification of interest.

Figure 4. Cross reactivity tests using the Diagenode antibody directed against H3K18ac
To test the cross reactivity of the Diagenode antibody against H3K18ac (cat. No. C15410139), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K18. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:5,000. Figure 4 shows a high specificity of the antibody for the modification of interest.

<strong> Figure 5. Western blot analysis using the Diagenode antibody directed against H3K18ac</strong><br /> Western blot was performed on whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the Diagenode antibody against H3K18ac (cat. No. C15410139). The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left.

Figure 5. Western blot analysis using the Diagenode antibody directed against H3K18ac
Western blot was performed on whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the Diagenode antibody against H3K18ac (cat. No. C15410139). The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left.

<strong> Figure 6. Immunofluorescence using the Diagenode antibody directed against H3K18ac</strong><br /> HeLa cells were stained with the Diagenode antibody against H3K18ac (cat. No. C15410139) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K18ac antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

Figure 6. Immunofluorescence using the Diagenode antibody directed against H3K18ac
HeLa cells were stained with the Diagenode antibody against H3K18ac (cat. No. C15410139) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K18ac antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.