Anti-Acetyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody, Clone RM172

Name	Anti-Acetyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody, Clone RM172
Supplier	RevMab Bioscience
Catalog	31-1056-00
Prices	$149.00, $349.00
Sizes	25 µg, 100 µg
Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG
Clone	RM172
Applications	WB ELISA Multiplex ICC/IF ChIP
Species Reactivities	Broad species
Antigen	An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys27).
Purity/Format	Protein A affinity purified from an animal origin–free culture supernatant
Description	Rabbit Monoclonal
Conjugate	None
Supplier Page	Shop

RM172 specifically reacts to Histone H3

acetylated at Lysine 27 (K27ac). No cross reactivity

with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac),

Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23

(K23ac), Lysine 36 (K36ac), Lysine 56 (K56ac),

Lysine 79 (K79ac), or Lysine 122 (K122) in Histone H3.

Western Blot of acid extracts from HeLa cells

untreated (-) or treated with sodium butyrate (+),

using RM172 at 1 ug/mL, showed a band of

histone H3 acetylated at Lysine 27 in treated

HeLa.

Immunocytochemistry of HeLa cells treated with

sodium butyrate, using Acetyl-Histone H3 (Lys27)

Rabbit mAb RM172 (red). Actin filaments have

been labeled with fluorescein phalloidin (green).