AR (441): sc-7305. Immunofluorescence staining of methanol-fixed T-47D cells (A) and immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue (B) showing nuclear localization.
AR siRNA (h): sc-29204. Western blot analysis of AR expression in non-transfected control (A) and AR siRNA transfected (B) MCF7 cells. Blot probed with AR (441): sc-7305. a-actinin (H-2): sc-17829 used as specificity and loading control.
Western blot analysis of AR expression in AT-3 (A), T-47D (B), ZR-75-1 (C,F) and MCF7 (D) whole cell lysates and SK-BR-3 (E) nuclear extract. Antibodies tested include AR (N-20): sc-816 (A-E) and AR (441): sc-7305 (F).
AR (441): sc-7305. Western blot analysis of AR expression in untreated (A) and Brefeldin A (BFA) (sc-200861) treated (B) LNCaP whole cell lysates. Note down regulation of AR expression in lane B.