Detection of Human and Mouse by Immunohistochemistry. Sample: FFPE sections of human ovarian carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- used at a dilution of 1:100 (2 Detection: DAB.
Detection of Human CTCF by Western Blot and Immunoprecipitation. Samples: A) Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CTCF antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. For blotting immunoprecipitated CTCF, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 minutes (B).
Detection of Mouse CTCF by Western Blot. Samples: Whole cell lysate (50 ug) from CT26.WT, 4T1, Renca, and TCMK-1 cells. Antibodies: Affinity purified rabbit anti-CTCF antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.