Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with E2F5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of E2F5 Antibody in HepG2 cell line lysates (35 ug/lane). E2F5 (arrow) was detected using the purified antibody.
E2F5 Antibody FC of ZR-75-1 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.