Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Confocal immunofluorescent of Phospho-HER4-Y1162 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Phospho-HER4-Y1162 Antibody was used to detect Phospho-HER4-Y1162 in FG pancreatic carcinoma cell line lysate. FG pancreatic carcinoma cells treated with or without EGF (50ng/ml) for 15 min Phospho-HER4-Y1162 Antibody was used at 1:750 in 3% BSA. Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.