Anti-KIAA0652 / ATG13 Antibody
| Name | Anti-KIAA0652 / ATG13 Antibody |
|---|---|
| Supplier | LifeSpan Bioscience |
| Catalog | LS-C153856 |
| Prices | $395.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ELISA |
| Species Reactivities | Human, Mouse, Rat |
| Antigen | This affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the S318 region of ATG13. |
| Purity/Format | Immunoaffinity purified |
| Description | Rabbit Polyclonal |
| Gene | ATG13 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Western blot of affinity purified anti-ATG13 antibody shows detection of ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13 (Ulk1 + Atg13), right lane, but not in the left lane in which was loaded kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13. Detection is demonstrated at approximately 57 kDa. The antibody was purified and resolved by SDS-PAGE, then transferred to nitrocellulose membrane. The membrane was blocked with 5% Blotto (p/n B501-0500) and probed with the primary antibody at 1g/mL overnight at 4C. After washing, the membrane was probed with Goat Anti-Rabbit HRP secondary 1:5000 in detection buffer (p/n MB-070) for 45 minutes at room temperature. In collaboration with Charles Dorsey at Eli Lilly, Indianapolis, IN and John Cleveland at Scripps, Jupiter, FL.