IHC of paraffin-embedded pancreas tissue using anti-FERMT2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Ovary tissue using anti-FERMT2 mouse monoclonal antibody. (Dilution 1:50).
Immunofluorescent staining of HepG2 cells using anti-FERMT2 mouse monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-FERMT2 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FERMT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FERMT2.
Immunoprecipitation(IP) of FERMT2 by using monoclonal anti-FERMT2 antibodies (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of DDK tagged FERMT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-FERMT2 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.