Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of anti-SKY antibody in mouse brain lysate. SKY (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot of SKY (arrow) using rabbit polyclonal SKY Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SKY gene (Lane 2) (Origene Technologies).