Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 4856
• Prices: $309.00
• Sizes: 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: 2F9
• Applications: WB ICC/IF FC
• Species Reactivities: Human, Mouse, Rat, Monkey
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6.
• Description: Rabbit Monoclonal
• Gene: RPS6

Product images

Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-treated (100 ng/ml, 20 min), using Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (upper) or S6 Ribosomal Protein (5G10) Rabbit mAb #2217 (lower).

Confocal immunofluorescent images of HeLa cells serum-starved for 20 hrs (left), 20% serum-treated (center), or 20% serum-treated after preincubation with Rapamycin (FRAP/mTOR Inhibitor) #9904 and labeled with Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Flow cytometric analysis of Jurkat cells, untreated (green), or LY294002, Wortmannin and U0126-treated (blue), using Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb compared to a nonspecific negative control antibody (red).