SR-BI Antibody

• Supplier: Novus Biologicals
• Catalog: NB400-104
• Prices: $129.00, $349.00
• Sizes: 25 µl, 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB Simple Western FC ICC/IF IHC IHC-F IHC-P IP
• Species Reactivities: Human, Rat, Rabbit
• Antigen: A C-terminal peptide containing residues from mouse SR-BI (within residues 450-509). [UniProt# Q61009]
• Purity/Format: Immunogen affinity purified
• Description: Rabbit Polyclonal
• Gene: Scarb1
• Conjugate: Unconjugated

Product images

Western Blot: SR-BI Antibody [NB400-104] - Detection of SR-BI in rat H4IIE total cell lysates and plasma membrane proteins. Photo courtesy of product review by verified customer.

Western Blot: SR-BI Antibody [NB400-104] - Analysis using the HRP conjugate of NB400-104. Detection of SR-BI in 25 ug of mouse liver lysate, using NB400-104 (lotJ) at a 1:1,000 dilution, shows distinct band at 82 kDa.

Flow Cytometry: SR-BI Antibody [NB400-104] - An intracellular stain was performed on HeLa cells with SR-BI antibody NB400-104AF488 (blue) and a matched isotype control NBP2-24893AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Immunocytochemistry/Immunofluorescence: SR-BI Antibody [NB400-104] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-SR-BI at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-104] - IHC analysis of a formalin fixed paraffin embedded tissue section of Mouse liver using SR-BI antibody (Lot R-4) at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated mainly a membranous signal of SR-BI protein in the murine hepatocytes.

Flow Cytometry: SR-BI Antibody [NB400-104] - An intracellular stain was performed on HeLa cells with SR-BI antibody NB400-104PE (blue) and a matched isotype control NBP2-24893PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Western Blot: SR-BI Antibody [NB400-104] - SR-BI antibody was tested in human adrenal cell lysate.

Western Blot: SR-BI Antibody [NB400-104] - Detection of HRP conjugated SR-BI in 25 ug of mouse liver lysate at a 1:1,000 dilution, shows distinct band at 82 kDa.

Immunohistochemistry: SR-BI Antibody [NB400-104] - Immunolocalization of SR-BI in adult mink testis using NB400-104. SR-BI labeling is visible at the surface and along the outline of the large vacuole. Photo courtesy of R.M. Pelletier, University of Montreal.

Immunohistochemistry: SR-BI Antibody [NB400-104] - Staining of adrenal cortex.

Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-104] - IHC analysis of a formalin fixed paraffin embedded tissue section of Mouse liver using SR-BI antibody (Lot 8310) at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a specific membrane signal of SR-BI protein in the murine hepatocytes.

Flow Cytometry: SR-BI Antibody [NB400-104] - Analysis of Huh7 and HepG2 cells using SR-B1 antibody NB400-104. Courtesy of Bruno Sainz, Jr., PhD, University of Illinois at Chicago.

Flow Cytometry: SR-BI Antibody [NB400-104] - Analysis using the DyLight 550 conjugate of NB400-104. SR-B1 staining of human adipose tissue macrophage populations compared to isotype control (orange). Image from verified customer review.

Flow Cytometry: SR-BI Antibody [NB400-104] - An intracellular stain was performed on HeLa cells with SR-BI antibody NB400-104AF647 (blue) and a matched isotype control NBP2-24893AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Simple Western: SR-BI Antibody [NB400-104] - Simple Western lane view shows a specific band for SR-BI in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.