![Western Blot: Laminin Antibody [NB300-144] - Analysis of rat heart cells lysates (lane 1) and 0.2 ug of purified laminin protein from mouse EHS sarcoma (lane 2) This antibody recognizes 3 laminin isotypes: alpha 1 (440kDa), beta 1 (220kD) and gamma 1 (220kDa). Also recognized is a laminin binding protein at 120kDa in both rat heart lysates and purified laminin protein. Since this protein always coexpresses with laminin this crossreactivity is irrelevant.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_6/25940_Laminin-Antibody-Western-Blot-NB300-144-img0017.jpg)
Western Blot: Laminin Antibody [NB300-144] - Analysis of rat heart cells lysates (lane 1) and 0.2 ug of purified laminin protein from mouse EHS sarcoma (lane 2) This antibody recognizes 3 laminin isotypes: alpha 1 (440kDa), beta 1 (220kD) and gamma 1 (220kDa). Also recognized is a laminin binding protein at 120kDa in both rat heart lysates and purified laminin protein. Since this protein always coexpresses with laminin this crossreactivity is irrelevant.
![Immunocytochemistry/Immunofluorescence: Laminin Antibody [NB300-144] - IF Confocal analysis of HeLa cells using Laminin antibody (NB300-144, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_6/25941_Laminin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-144-img0011.jpg)
Immunocytochemistry/Immunofluorescence: Laminin Antibody [NB300-144] - IF Confocal analysis of HeLa cells using Laminin antibody (NB300-144, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).
![Immunohistochemistry: Laminin Antibody [NB300-144] - Staining of mouse section of cortex stained with Laminin- (red). Blue is DAPI staining of DNA. This antibody reveals strong staining in the basement membranes of blood vessels.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_6/25942_Laminin-Antibody-Immunohistochemistry-NB300-144-img0014.jpg)
Immunohistochemistry: Laminin Antibody [NB300-144] - Staining of mouse section of cortex stained with Laminin- (red). Blue is DAPI staining of DNA. This antibody reveals strong staining in the basement membranes of blood vessels.
![Western Blot: Laminin Antibody [NB300-144] - Analysis of Laminin-1 expression in mouse EHS tumor crude extracts (left) and Laminin-2 expression in rat heart crude extracts (right). The Laminin polyclonal antibody was used at 1 ug/ml.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_6/25943_Laminin-Antibody-Western-Blot-NB300-144-img0010.jpg)
Western Blot: Laminin Antibody [NB300-144] - Analysis of Laminin-1 expression in mouse EHS tumor crude extracts (left) and Laminin-2 expression in rat heart crude extracts (right). The Laminin polyclonal antibody was used at 1 ug/ml.
![Immunocytochemistry/Immunofluorescence: Laminin Antibody [NB300-144] - Detection of Laminin (Green) in Hela cells using NB300-144 at a 1:50 dilution. Nuclei (Blue) were counterstained using Hoechst 33258.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_6/25944_Laminin%2520Antibody-Immunocytochemistry-Immunofluorescence-NB300-144-img0001.jpg)
Immunocytochemistry/Immunofluorescence: Laminin Antibody [NB300-144] - Detection of Laminin (Green) in Hela cells using NB300-144 at a 1:50 dilution. Nuclei (Blue) were counterstained using Hoechst 33258.
![Immunohistochemistry: Laminin Antibody [NB300-144] - Staining of rat spinal cord and dorsal root paraformaldehyde/paraffin-embedded tissue using NB 300-144. Pepsin antigen retrieval was performed on this tissue sample.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_6/25945_Laminin%2520Antibody-Immunohistochemistry-NB300-144-img0008.jpg)
Immunohistochemistry: Laminin Antibody [NB300-144] - Staining of rat spinal cord and dorsal root paraformaldehyde/paraffin-embedded tissue using NB 300-144. Pepsin antigen retrieval was performed on this tissue sample.